Figure 1.

Immunization of B6 mice with RMA-S.B7–1 cells elicits CD8⁺ CTLs that recognize nontransfected RMA-S cells. (A and B) B6 mice were immunized in vivo and splenocytes were restimulated in vitro with RMA-S cells (○) or RMA-S cells transfected with B7–1 (⧫) and tested for cytotoxicity against RMA-S target cells. Two experiments are shown; A is representative of 4/6 experiments, B is representative of the remaining two experiments. (C) CTLs generated as above were depleted with anti-CD8 antibodies and complement (▴) or anti-CD4 antibodies and complement (▵) and tested for cytotoxicity against RMA-S target cells. One representative experiment of three is shown.