Figure 4.

(A) Regional expression profiles, determined by RT-PCR, in naive adult head (H), thorax (T), midgut (G), and remaining abdominal tissues (R; which includes hindgut, Malphigian tubules and ovaries). ISP13 is largely amplified from the gut cDNA but also shows lower level expression in the head and abdomen. ISPL5 is mainly amplified in the thorax and abdomen. GNBP is mainly amplified from the thorax, defensin from the thorax, midgut and abdomen, and IGALE20 from the midgut. (B) Specific expression of the markers in anterior (A) versus posterior (P) regions of the unchallenged midgut (shown in a photograph at the top). ISP13 is posterior midgut specific and defensin is limited to the anterior midgut. ISPL5, GNBP and IGALE20 amplify weakly, approximately equally from both posterior and anterior midguts.