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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Opt Commun. 2008 Apr 1;281(7):1806–1812. doi: 10.1016/j.optcom.2007.05.070

Fig. 2.

Fig. 2

Effect of correction collar adjustments on the point spread functions of fluorescent microspheres. XZ cross section of 0.5 micron fluorescent microspheres mounted immediately below the coverslip with collar settings (A) 0.13, (B) 0.17, and (C) 0.21 mm. XZ cross section of 0.5 micron fluorescent microspheres mounted below 50 microns of fixed kidney tissue with collar settings (D) 0.13, (E) 0.17, and (F) 0.21 mm. Pseudocolor based on intensity. Scalebar = 2 microns, x=z.