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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Opt Commun. 2008 Apr 1;281(7):1806–1812. doi: 10.1016/j.optcom.2007.05.070

Fig. 3.

Fig. 3

Effect of correction collar adjustment on fluorescence intensity as a function of depth in kidney tissue. Multiphoton fluorescence images of nuclei labeled with Hoechst 33342 were collected at various depths from the kidney of a living rat, using collar settings of 0.14 (▲), 0.17 (○), 0.19 mm (□). Mean fluorescence intensity of each plane is plotted, normalized to the peak of each plot. Curves included to guide the eye.