Table 1.
A comparison of biological samples available for studying cancer proteome
| Cancer cell line | Human tissue | Body fluids/plasma | |
|---|---|---|---|
| Advantages | Low cost | High physiological/pathological relevance | Low cost |
| Renewable source | High physiological/pathological relevance | ||
| Easy for gene manipulation | Easy to obtain from patients/ renewable source | ||
| Good for mechanistic investigation | |||
| Disadvantages | Require validation for physiological/pathological relevance | Higher cost | Higher protein complexity |
| Clonal selection | Limited availability | High dynamic range in protein concentrations | |
| Non‐renewable source | |||
| Large variations among samples |