Figure 3.

Expression of pre-pro-MIC-1 cDNA in 293-EBNA cells. Supernatant (A) and total cell lysate (B) from untransfected (a) and MIC-1 transfected (b) 293-EBNA cells were immunoprecipitated with anti-FLAG antibodies and analyzed by immunoblotting with anti-FLAG antibodies under both reducing (R) and nonreducing (NR) conditions. The various forms of MIC-1 corresponding to the indicated bands are depicted on the right, with the closed bar indicating the pro-domain and the open bar indicating the mature domain.