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MIC-1 inhibits TNF-α production by macrophages. Macrophages were stimulated with LPS, and the effects on secretion of TNF-α were quantitated as the mean and standard deviation of triplicate cultures. Representative experiments showing the effect of MIC-1 or TGF-β1 (A) and of the preincubation period with MIC-1 or TGF-β1 (10 ng/ml) (B) on suppression of TNF-α secretion.
