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. 1997 Sep 30;94(20):10919–10924. doi: 10.1073/pnas.94.20.10919

Figure 1.

Figure 1

Targeted disruption of the Hmox1 gene. (a) Hmox1 genomic locus and targeting vector. A 3.7-kb region including exons 3, 4, and a portion of 5 (e3–e5) was replaced with a pgk-neo cassette. The 5′ and 3′ probes used for screening ES cell clones and genotyping mice are shown. The 5′ probe hybridizes to an 11-kb KpnI fragment of the native Hmox1 gene and a 6.5-kb fragment from the disrupted gene. D, HindIII site; K, KpnI; X, XhoI; Xb, XbaI. (b) Southern blot analysis of KpnI-digested tail DNA from ES cell-derived mice. The blot was hybridized with the 5′ Hmox1 probe. Genotypes of one Hmox1 homozygous mutant mouse (−/−), two wild-type mice (+/+), and two heterozygous mice (+/−) are indicated. (c) Northern blot analysis of total splenic RNA from a wild-type mouse (+/+), a heterozygous mouse (+/−), and a homozygous mutant mouse (−/−). The blot was hybridized with a rat Hmox1 cDNA probe (13), which recognizes a major mRNA band of approximately 1.5 kb. The Hmox1 probe recognized an aberrantly sized mRNA from Hmox1−/− mice that was barely detectable even in an overloaded lane.