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. 1977 Jun;130(3):1393–1396. doi: 10.1128/jb.130.3.1393-1396.1977

Deoxyribonucleic acid strand breaks during freeze-drying and their repair in Escherichia coli.

T Ohnishi, Y Tanaka, M Yoh, Y Takeda, T Miwatani
PMCID: PMC235372  PMID: 324987

Abstract

Freeze-drying of Escherichia coli cells caused strand breaks of deoxyribonucleic acid (DNA) in both radiation-sensitive and -resistant strains. However, in the radiation-resistant strain E. coli B/r the damaged DNA was repaired after rehydration, whereas in the radiation-sensitive strain E. coli Bs-1 the damaged DNA was not repaired and the DNA was degraded. Repeated freeze-drying did not break the damaged DNA into smaller pieces.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Braun A., Grossman L. An endonuclease from Escherichia coli that acts preferentially on UV-irradiated DNA and is absent from the uvrA and uvrB mutants. Proc Natl Acad Sci U S A. 1974 May;71(5):1838–1842. doi: 10.1073/pnas.71.5.1838. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. HILL R. F. A radiation-sensitive mutant of Escherichia coli. Biochim Biophys Acta. 1958 Dec;30(3):636–637. doi: 10.1016/0006-3002(58)90112-4. [DOI] [PubMed] [Google Scholar]
  3. HILL R. F., SIMSON E. A study of radiosensitive and radioresistant mutants of Escherichia coli strain B. J Gen Microbiol. 1961 Jan;24:1–14. doi: 10.1099/00221287-24-1-1. [DOI] [PubMed] [Google Scholar]
  4. Harm W. The role of host-cell repair in liquid-holding recovery of u.v.-irradiated Escherichia coli. Photochem Photobiol. 1966 Sep;5(9):747–760. doi: 10.1111/j.1751-1097.1966.tb05824.x. [DOI] [PubMed] [Google Scholar]
  5. Kondo S., Ichikawa H., Iwo K., Kato T. Base-change mutagenesis and prophage induction in strains of Escherichia coli with different DNA repair capacities. Genetics. 1970 Oct;66(2):187–217. doi: 10.1093/genetics/66.2.187. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Mattern I. E., Zwenk H., Rörsch A. The genetic constitution of the radiation-sensitive mutant Escherichia coli Bs-1. Mutat Res. 1966 Oct;3(5):374–380. doi: 10.1016/0027-5107(66)90047-9. [DOI] [PubMed] [Google Scholar]
  7. McGrath R. A., Williams R. W. Reconstruction in vivo of irradiated Escherichia coli deoxyribonucleic acid; the rejoining of broken pieces. Nature. 1966 Oct 29;212(5061):534–535. doi: 10.1038/212534a0. [DOI] [PubMed] [Google Scholar]
  8. SETLOW R. B., CARRIER W. L. THE DISAPPEARANCE OF THYMINE DIMERS FROM DNA: AN ERROR-CORRECTING MECHANISM. Proc Natl Acad Sci U S A. 1964 Feb;51:226–231. doi: 10.1073/pnas.51.2.226. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Servín-Massieu M., Cruz-Camarillo R. Variants of Serratia marcescens induced by freeze-drying. Appl Microbiol. 1969 Oct;18(4):689–691. doi: 10.1128/am.18.4.689-691.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sinskey T. J., Silverman G. J. Characterization of injury incurred by Escherichia coli upon freeze-drying. J Bacteriol. 1970 Feb;101(2):429–437. doi: 10.1128/jb.101.2.429-437.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Tanaka Y., Ohnishi T., Takeda Y., Miwatani T. Lethal effect of freeze-drying on radiation-sensitive mutants of Escherichia coli. Biken J. 1975 Dec;18(4):267–269. [PubMed] [Google Scholar]
  12. Tanaka Y., Onishi T., Arita M., Takeda Y., Miwatani T. [Disturbance of DNA of Escherichia coli by freeze drying]. Nihon Saikingaku Zasshi. 1975 Jan;30(1):185–185. [PubMed] [Google Scholar]
  13. Yonei S., Nozu K. Mechanism of post-irradiation degradation of deoxyribonucleic acid in a radiosensitive Escherichia coli (NG30) irradiated with ultraviolet light. J Mol Biol. 1972 Mar 28;65(2):213–225. doi: 10.1016/0022-2836(72)90278-1. [DOI] [PubMed] [Google Scholar]

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