Figure 2.

Southern blot analysis of representative M. tuberculosis::Tn5368 clones and expected schematic hybridization patterns for a transposition mutant. Five mutants were picked at random (clones 1–5). M. tuberculosis 103 DNA (WT) was included as a control and as expected showed no hybridization signal. Genomic DNA was digested with BamHI or XhoI and probed for hybridization with pPR32, a vector consisting of the Tn5368 transposon cloned into the BamHI site of pPR23. Molecular masses are indicated in kilobases.