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. Author manuscript; available in PMC: 2009 May 1.
Published in final edited form as: Protein Expr Purif. 2008 Feb 5;59(1):144–152. doi: 10.1016/j.pep.2008.01.016

Fig. 3.

Fig. 3

Coexpression enhances the stability of full-length human JunB expressed in E. coli. Bacterial lysate and sonication supernatant taken from individually expressed F:JunB or coexpressed F:JunB/6His:c-Fos were separated by 10% SDS-PAGE and analyzed by Western blotting with anti-FLAG M2 monoclonal antibody. Lanes 1 and 2, F:JunB expressed from a monocistronic plasmid, pF:JunB-11d, which carries the coding sequence of FLAG-tagged human JunB. Lanes 3 and 4, full-length F:JunB stabilized by coexpressed 6His:c-Fos.