Figure 4.

The TEF protein bound specifically to a subgroup E SU-immunoadhesin. Protein A-Sepharose beads bound to either SUB or SUE immunoadhesin were incubated with protein lysates from 293 cells transfected with either plasmid pBKTEF24 or plasmid pBK7.6–2 expressing CAR1 or with no DNA (mock). The immunoprecipitated protein was subjected to SDS/polyacrylamide gel electrophoresis and immunoblotted with the IQS579 CAR1 peptide antibody.