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. 1997 Oct 14;94(21):11627–11632. doi: 10.1073/pnas.94.21.11627

Table 1.

Reversible inhibition of [3H]InsP3 binding by calmodulin

Calmodulin, μM Inhibition of [3H]InsP3 binding, %
 3 54  ±  1
10 72  ±  2
10 then 3 49  ±  1

SPA methods were used to compare [3H]InsP3 binding to receptors incubated with 3 or 10 μM calmodulin for 40 min. In parallel, receptors were incubated with 10 μM calmodulin (20 min) and then incubated for a further 20 min after dilution to reduce the calmodulin concentration to 3 μM without otherwise changing the composition of the medium. The results (means ± SEM, n = 6) are expressed relative to the specific [3H]InsP3 binding detected in parallel incubations without calmodulin.

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