Abstract
Induction of lactose transport and of beta-galactosidase synthesis was examined in two Escherichia coli strains that require exogenous glycerol for phospholipid synthesis and growth. No preferential inhibition of lactose transport induction was observed when phospholipid synthesis was restricted to 5 to 10% of the normal rate. We conclude that the lactose transport system does not require concurrent phospholipid synthesis for its functional assembly.
Full text
PDF




Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bell R. M. Mutants of Escherichia coli defective in membrane phospholipid synthesis: macromolecular synthesis in an sn-glycerol 3-phosphate acyltransferase Km mutant. J Bacteriol. 1974 Mar;117(3):1065–1076. doi: 10.1128/jb.117.3.1065-1076.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cronan J. E., Jr, Bell R. M. Mutants of Escherichia coli defective in membrane phospholipid synthesis: mapping of sn-glycerol 3-phosphate acyltransferase Km mutants. J Bacteriol. 1974 Oct;120(1):227–233. doi: 10.1128/jb.120.1.227-233.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cronan J. E., Jr, Bell R. M. Mutants of Escherichia coli defective in membrane phospholipid synthesis: mapping of the structural gene for L-glycerol 3-phosphate dehydrogenase. J Bacteriol. 1974 May;118(2):598–605. doi: 10.1128/jb.118.2.598-605.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fox C. F. A lipid requirement for induction of lactose transport in Escherichia coli. Proc Natl Acad Sci U S A. 1969 Jul;63(3):850–855. doi: 10.1073/pnas.63.3.850. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hsu C. C., Fox C. F. Induction of the lactose transport system in a lipid-synthesis-defective mutant of Escherichia coli. J Bacteriol. 1970 Aug;103(2):410–416. doi: 10.1128/jb.103.2.410-416.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mindich L. Induction of Staphylococcus aureus Lactose Permease in the Absence of Glycerolipid Synthesis. Proc Natl Acad Sci U S A. 1971 Feb;68(2):420–424. doi: 10.1073/pnas.68.2.420. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nunn W. D., Cronan J. E., Jr Unsaturated fatty acid synthesis is not required for induction of lactose transport in Escherichia coli. J Biol Chem. 1974 Feb 10;249(3):724–731. [PubMed] [Google Scholar]
- Overath P., Hill F. F., Lamnek-Hirsch I. Biogenesis of E. coli membrane: evidence for randomization of lipid phase. Nat New Biol. 1971 Dec 29;234(52):264–267. doi: 10.1038/newbio234264a0. [DOI] [PubMed] [Google Scholar]
- Putnam S. L., Koch A. L. Complications in the simplest cellular enzyme assay: lysis of Escherichia coli for the assay of beta-galactosidase. Anal Biochem. 1975 Feb;63(2):350–360. doi: 10.1016/0003-2697(75)90357-7. [DOI] [PubMed] [Google Scholar]
- Willecke K., Mindich L. Induction of citrate transport in Bacillus subtilis during the absence of phospholipid synthesis. J Bacteriol. 1971 May;106(2):514–518. doi: 10.1128/jb.106.2.514-518.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]