Figure 2.

Activation by LTP of GAP-43 promoter activity in dentate gyrus of GAP-43/lacZ transgenic mouse. (A and B) Arrowheads point to LTP-induced X-Gal staining of β-galactosidase activity in the inner molecular layer (IML) just external to the hippocampal granule cell layer (GCL) in transgenic mice. (C and D) Lack of induction of PATE in IML in LFC animals. Note greater number of X-Gal-stained cells (P < 0.05) were observed in the ipsilateral hilus (H) of the LTP group (A), compared with the contralateral side (B) and LFC animals (C and D). No X-Gal staining was observed in the granule cell layer (GCL) or their axons, the mossy fibers in either the LTP or the LFC group. Note that in the central hilar region, the ipsilateral side of the LTP group showed stronger staining intensity as well as increased numbers of stained cells compared with the contralateral side or either side of the LFC group. (Scale bar in A = 50 μm.)