Figure 5.

Representative flow cytometer plots of liver endothelial cells 30 min after intravenous injection of buffer [10 mM N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid (Hepes)/135 mM NaCl, pH 6.7] (control) or DiI-labeled Aco-HSA liposomes. DiI-labeled liposomes (1 μmol of total lipid/100 g body weight) or buffer were injected into anesthetized rats. Endothelial cells were isolated and subjected to flow cytometry as described in Materials and Methods. Fluorescence at 525 nm is depicted on a log scale.