Table 1.
Effect of the size of Aco-HSA liposomes on the uptake by liver endothelial cells and Kupffer cells 30 min after intravenous injection
| Pore size, nm* | Uptake, μmol total lipid/1⋅106 cells
|
|
|---|---|---|
| Endothelial cells | Kupffer cells | |
| 50 | 2.8 ± 0.5† | 3.3 ± 0.5† |
| 100 | 1.9 ± 0.1 | 7.4 ± 0.0 |
| 200 | 1.0 ± 0.1 | 15.7 ± 1.9 |
| 400 | 1.0 ± 0.1 | 17.6 ± 2.2 |
[3H]cholesteryloleyl-ether labeled liposomes (1 μmol of total lipid/100 g body weight), extruded through filters with the indicated pore size, were injected into anesthetized rats. Intrahepatic distribution was determined as described in Materials and Methods. Data are presented as means ± half the difference between the two values of two rats or
†
as means ± SD of three rats.
*
Actual average diameters after extrusion through filters with the indicated pore size were 92.1 nm (50 nm), 153.5 nm (100 nm), 263.2 nm (200 nm), and 590.7 nm (400 nm).