Table 2. Inhibition of longipain by various proteinase inhibitors.

Inhibitor	Concentration (mM)	Inhibition (%) relative to control
		Z-R-R-MCA	Z-F-R-MCA
E-64	0.01	10.0±0.1	84.0±0.2
Antipain	0.05	0	0
Leupeptin	0.10	0	0
PepstatinA	0.10	0	4.1±0.01
PMSF	10.0	20.1±0.1	0

The purified longipain was incubated with fluorogenic substrates in the presence of the protease inhibitors at the indicated concentration. The inhibitory effect is indicated as the percentage of the maximum hydrolytic activity of longipain for each substrate. Data shown are the mean±S.D. (n = 3).