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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Toxicol Appl Pharmacol. 2007 Dec 15;228(1):49–58. doi: 10.1016/j.taap.2007.12.010

Fig. 5.

Fig. 5

Protective effect of dehydroascorbic acid (DHA) and L-ascorbic acid 6-palmitate (AA6P) from fullerol phototoxicity in human lens epithelial cells. The cells were incubated with an ascorbate derivative in Minimum Essential Medium (MEM)/DMSO (99:1) for 2 h and with 20 µM fullerol in MEM/DMSO (99:1) for the next 16 h. Then the cells were washed and overlaid with HBSS and exposed to UVA for 10 min. After irradiation, the cells were incubated overnight in MEM/FBS (8:2). Metabolic activity (A, C) and lactate dehydrogenase release (B, D) from the cells were determined with MTS and LDH assays, respectively. Results are expressed as mean ± SE (n=4). * p<0.01, and ** p<0.02 comparing metabolic activity or LDH release in cells incubated with antioxidant and fullerol vs. cells incubated only with fullerol both in the “dark” and “UVA exposed” group.