Figure 5. EFL-1 and LIN-35 have opposite effects on lip-1 mRNA levels.
lip-1 antisense RNA probes were used for in situ hybridization of dissected gonads. Two gonads are shown in the efl-1(RNAi) panel. Asterisk indicates the distal end of gonad. All genotypes were stained in parallel with identical hybridization, color development, and image exposure times. (B) Two EFL-1/DPL-1 consensus motifs are present upstream of the lip-1 translational start site, at −282 bp and −2017 bp, and one candidate motif previously found upstream of LIN-35 downregulated genes is located at −964 bp.