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. Author manuscript; available in PMC: 2009 Apr 2.
Published in final edited form as: DNA Repair (Amst). 2008 Feb 12;7(4):558–571. doi: 10.1016/j.dnarep.2007.12.010

Table 1.

Strains used in this study

Strain Genotype
W1588-4C MATa ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 RAD5
W1588-4A MATα ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 RAD5
J276 W1588-4A sgs1-K706R
J730 W1588-4C sgs1-K706A
J734 W1588-4A sgs1-NΔ82
J735 W1588-4A sgs1-NΔ82,K706R
J739 W1588-4A SGS1-3HA
J740 W1588-4A sgs1-K706R-3xHA
J741 W1588-4A sgs1-K706A-3xHA
J742 W1588-4A sgs1-NΔ82-3xHA
J743 W1588-4A sgs1-NΔ82,K706R-3xHA
W1874 Diploid wild-type (W1588-4C/W1588-4A)
W1956 Diploid sgs1::URA3/SGS1 top1::HIS3/TOP1
W1958 Diploid sgs1::HIS3/SGS1 top3::TRP1/TOP3
W1857 Diploid sgs1::URA3/SGS1 srs2::HIS3/SRS2
W1914 Diploid sgs1-K706R/SGS1 srs2::HIS3/SRS2
W2008 Diploid sgs1-K706A/SGS1 srs2::HIS3/SRS2
W2087 Diploid sgs1-NΔ82/SGS1 srs2::HIS3/SRS2
W2088 Diploid sgs1-NΔ82,K706R/SGS1 srs2::HIS3/SRS2
W1875 Diploid sgs1::HIS3/sgs1::HIS3
W1949 Diploid top3::TRP1/top3::TRP1
W1950 Diploid sgs1-K706R/sgs1-K706R
W2034 Diploid sgs1-K706A/sgs1-K706A
W1911 Diploid sgs1-K706R/SGS1
W2036 Diploid sgs1-K706A/SGS1
W2069 Diploid sgs1-NΔ82/SGS1
W2075 Diploid sgs1-NΔ82,K706R/SGS1
W2065 Diploid sgs1-NΔ82/SGS1top1::HIS3/TOP1
W2071 Diploid sgs1-NΔ82,K706R/SGS1top1::HIS3/TOP1
W2066 Diploid sgs1-NΔ82/SGS1top3::TRP1/TOP3
W2072 Diploid sgs1-NΔ82,K706R/SGS1top3::TRP1/TOP3
W2068 Diploid sgs1-NΔ82/SGS1 srs2::HIS3/SRS2
W2074 Diploid sgs1-NΔ82,K706R/SGS1 srs2::HIS3/SRS2
W1959 Diploid sgs1::HIS3/SGS1 top3::TRP1/TOP3 SUP4-o::URA3
W1934 Diploid sgs1-K706R/SGS1 top3::TRP1/TOP3 SUP4-o::URA3
W2082 Diploid sgs1-NΔ82/SGS1 SUP4-o::URA3
W2083 Diploid sgs1-NΔ82,K706R/SGS1 SUP4-o::URA3
W2089 Diploid sgs1-NΔ82/SGS1 mec1::TRP1/MEC1 sml1-1/SML1
W2090 Diploid sgs1-NΔ82,K706R/SGS1 mec1::TRP1/MEC1 sml1-1/SML1
W2094 Diploid sml1::HIS3/SML1 mec1::TRP1/MEC1 sgs1::URA3/SGS1 top3::LEU2/TOP3
W2706 Diploid sgs1::URA3/SGS1 srs2::HIS3/SRS2 rad55Δ/RAD55
W2722 Diploid top3::TRP1/TOP3 srs2::HIS3/SRS2 rad55Δ/RAD55
W2725 Diploid sgs1-NΔ82/SGS1 srs2::HIS3/SRS2 rad55Δ/RAD55
W2726 Diploid sgs1-NΔ82,K706R/SGS1 srs2::HIS3/SRS2 rad55Δ/RAD55
W3058 Diploid sgs1-K706R/SGS1 srs2::HIS3/SRS2 rad51::LEU2/RAD51

All strains are RAD5 isogenic derivatives of W303 [56, 57]