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. 2008 Mar 18;98(7):1217–1225. doi: 10.1038/sj.bjc.6604289

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Copyright © 2008 Cancer Research UK

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Effect of whole or F(ab’)₂ anti-CD16 on hPR1A3-mediated lysis. Fluorescence-based ADCC assays were done using human PBMCs as effector cells in a ratio of 100 : 1 with MKN45 target cells. Columns represent mean % lysis from triplicate wells containing both target and effector cells ±10 μg ml⁻¹ hPR1A3 ±second antibody as indicated. The anti-CD16 constructs and anti-PSMA control were added at the same time as the effector cells without prior incubation.