Figure 7.

(A) Demonstration of NK cell purification. NK cells were purified from a single donor buffy coat sample (NBS) using the Human NK isolation kit. FACS analysis was performed using CD56 and CD16 antibodies pre- and post-sorting to show the enrichment of NK cells, which are both CD16- and CD56-positive. (B) Purified NK cells are much more effective killers than unfractionated PBMC. PBMC and NK cells were isolated from a single donor buffy coat (NBS). SKCO-1 was used as the target in the presence of PBMC (△) or NK cells (◊) with PR1A3 (50 μg ml⁻¹), or with no antibody (PBMC, ▪; NK, ▽). The flouresence-based EuTDA assay was used with effector:target ratios ranging from 10 : 1 to 50 : 1.