Table 1. Primers used for the EGFR gene mutation analysis for the first and the second PCR.

	Primers
EGFR gene	Forward	Reverse
Exon 18
First PCR	GCATGGTGAGGGCTGAGGTGA	CCCCACCAGACCATGAGAGGC
Second PCR	ACCCTTGTCTCTGTGTTCTTGTCCC	GCCCAGCCCAGAGGCCTGTG
Exon 19
First PCR	TGCCAGTTAACGTCTTCCTTC	CCACACAGCAAAGCAGAAAC
Second PCR	AACGTCTTCCTTCTCTCTCTG	CCACACAGCAAAGCAGAAAC
Exon 20
First PCR	CCACCATGCGAAGCCACACTGA	TCCTTATCTCCCCTCCCCGTATCTC
Second PCR	CCATGCGAAGCCACACTGACGT	CCCCTCCCCGTATCTCCCTTCC
Exon 21
First PCR	AGCTTCTTCCCATGATGATCTGTCC	GGCAGCCTGGTCCCTGGTGTC
Second PCR	TCCCATGATGATCTGTCCCTCACA	CAGGAAAATGCTGGCTGACCTAAAG

EGFR=epidermal growth factor receptor; PCR=polymerase chain reaction.