Figure 3.

MVA infection induced rapid BMDC maturation. Immature BMDCs were mock treated, infected with rMVA-GFP at a MOI of 10, or stimulated with 10 μg/ml LPS as described in Methods. At 0, 12 and 18 h post treatment, cells were harvested and subjected to cell surface staining for the indicated maturation markers. Samples were analyzed by flow cytometry. Histograms were gated on GFP⁺ cells. These data are representative of five independent experiments.