Table 1.
QPCR assays. QPCR assays (Assays on Demand) were from Applied Biosystems (Palo Alto, CA). Reverse primers from each assay were used for the synthesis of gene-specific cDNAs. They were provided separately by Applied Biosystems. Three assays (IGBP5_short, IGBP5_medium, IGBP5_long) were designed manually.
| AoD | Assay | Acc_Nr | AmpliconSize | Module |
| Hs00608023_m1 | BCL2 | NM_000633 | 81 | Estrogen |
| Hs00221277_m1 | CEGP1 | NM_020974 | 64 | Estrogen |
| Hs00174860_m1 | ESR1 | NM_000125 | 62 | Estrogen |
| Hs00172183_m1 | PGR | NM_000926 | 118 | Estrogen |
| Hs00180450_m1 | GRB7 | NM_005310 | 70 | Her2 |
| Hs01001598_g1 | HER2 | NM_004448 | 55 | Her2 |
| Hs00952036_m1 | CTSL2 | NM_001333 | 72 | Invasion |
| Hs00171829_m1 | STMY3 | NM_005940 | 66 | Invasion |
| Hs01030097_m1 | CCNB1 | NM_031966 | 66 | Proliferation |
| Hs01032443_m1 | MKI67 | NM_002417 | 66 | Proliferation |
| Hs00231158_m1 | MYBL2 | NM_002466 | 81 | Proliferation |
| Hs00269212_m1 | STK15 | NM_003600 | 85 | Proliferation |
| Hs00153353_m1 | SURV | NM_001168 | 93 | Proliferation |
| Hs99999903_m1 | ACTB | NM_001101 | 171 | Reference |
| Hs00266705_g1 | GAPDH | NM_002046 | 74 | Reference |
| Hs99999908_m1 | GUSB | NM_000181 | 81 | Reference |
| Hs99999902_m1 | RPLP0 | NM_001002 | 105 | Reference |
| Hs00174609_m1 | TFRC | NM_003234 | 79 | Reference |
| Hs00430290_m1 | UBB | NM_018955 | 120 | Reference |
| Hs01630490_s1 | RPL7A | BX641050 | 84 | Reference |
| Hs00817975_g1 | RPS11 | NM_001015 | 168 | Reference |
| Hs01922548_s1 | RPS23 | NM_001025 | 90 | Reference |
| Hs00185390_m1 | BAG1 | NM_004323 | 58 | |
| Hs00154355_m1 | CD68 | NM_001251 | 68 | |
| Hs01383449_s1 | GSTM1 | AY532925 | 65 | |
| (own design) | IGBP5_short | NM_000599 | 60 | Test |
| (own design) | IGBP5_medium | NM_000599 | 109 | Test |
| (own design) | IGBP5_long | NM_000599 | 147 | Test |