Table 1.
Summary of the primers designed and used in the study.
| Gene | Forward primer | Reverse Primer | Amplicon length | mRNA location |
| Human Prox1 (Exon 3–4) | 5'-GCT CCA ATA TGC TGA AGA CC-3' | 5'-ATC GTT GAT GGC TTG ACG TG-3' | 120 bp | 2399–2519 |
| Human HNF4α | 5'-GGG GTA CCC ATG GCC GAC TAC AGT G-3' | 5'-GGG GTA CCG CGC TGA CAC CCA GGC TG-3' | 140 bp | 122 – 262 |
| Human albumin | 5'-CTG TGG GGC ACT TTT GAA AT-3' | 5'-AAC ATC CCC TTC ACA GCA TC-3' | 154 bp | 698 – 851 |
| Human CREB | 5'-GAC ACC AGC TGG AGA AGG AC-3' | 5'-CTT CTT GGT GTC CCA TGC TT-3' | 198 bp | 500 – 698 |
| Human AP-4 | 5'-GTG CCC TCT TTG CAA CAT TT-3' | 5'-CTG CCT TGC TGA GCT TCT CT-3' | 229 bp | 289 – 517 |
| Human N-myc | 5'-GAC TGT AGC CAT CCG AGG AC-3' | 5'-GTT TTA ATA CCG GGG GTG CT-3' | 161 bp | 92 – 253 |
| Human MAZ | 5'-TAC CAC CTG AAC CGA CAC AA-3' | 5'-GCT GCC TCA CAT TTC TCA CA-3' | 247 bp | 1040 – 1287 |