Skip to main content
. 2008 Apr 23;105(17):6308–6313. doi: 10.1073/pnas.0707601105

Fig. 2.

Fig. 2.

p6-TfR is trafficked to the lumen of late endocytic compartments. (A) Cells transfected with p6-TfR-GFP (a), myc-p6-TfR (b), or TfR-GFP (c) were immunolabeled for EM, using antibodies to GFP or myc. Examples of nanogold-enhanced particles labeling endosomes are indicated by arrowheads. (Scale bars, 0.4 μm.) (B) (Left) Cells transfected with myc-p6-TfR were pulse-chased with Alexa594Tf, then fixed and stained with anti-myc. Arrows show examples of colocalization. Asterisks indicate nontransfected cells. (Scale bar, 10 μm.) (Right) Transfected cells treated as above were imaged by wide-field microscopy. Average Tf fluorescent intensities were calculated for each cell [nontransfected (cont) 124 cells; myc-TfR 40 cells; myc-p6-TfR 45 cells]. Values are means ± SEM.