Table 1.
Molecular dissection of HD-PTP function
| Construct | n | WT EGF |
Clustered EGF |
||
|---|---|---|---|---|---|
| Trans | Untrans | Trans | Untrans | ||
| FL HD-PTP | 6 | 99 ± 2 | 18 ± 5 | 1 ± 2 | 82 ± 5 |
| L202 | 4 | 88 ± 8 | 25 ± 12 | 12 ± 8 | 75 ± 12 |
| L202/I206 | 4 | 91 ± 7 | 28 ± 13 | 9 ± 7 | 72 ± 13 |
| Bro1 | 4 | 17 ± 5 | 9 ± 3 | 83 ± 5 | 91 ± 3 |
| Bro1-V | 7 | 85 ± 11 | 14 ± 5 | 15 ± 11 | 86 ± 5 |
| Bro1-V L202 | 3 | 77 ± 15 | 11 ± 3 | 23 ± 15 | 89 ± 3 |
| Bro1-V L202/I206 | 3 | 43 ± 6 | 7 ± 7 | 57 ± 6 | 93 ± 7 |
| ΔBro1 | 2 | 22 | 17 | 78 | 83 |
Cells depleted of HD-PTP were transfected with RNAi-resistant full-length (FL) HA-tagged HD-PTP or the indicated mutant. Bro1 refers to the Bro1 domain alone, and Bro1-V to the Bro1 and V domains alone. Cells were scored for the accumulation of fluorescent EGF in clusters or WT patterns of residual EGF staining. Neighboring untransfected cells (Untrans) from each sample were also scored. The number of experimental repeats is indicated. Values are means ± SD where appropriate.