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. 2008 Apr 21;105(17):6392–6397. doi: 10.1073/pnas.0802047105

Fig. 2.

Fig. 2.

Hypoxia-mediated EMT requires Notch. (A) Quantitative PCR analysis of E-cadherin mRNA expression in SKOV-3 cells kept at normoxia or treated with hypoxia for the indicated time (Left). Values represent average values of three independent experiments and are significant at **, P < 0.01 and *, P < 0.05 as indicated. Western blot analysis of E-cadherin protein levels in SKOV-3 cells cultured at normoxia or hypoxia in the absence or presence of GSI (Upper Right) or in SKOV-3 cells transfected with empty vector (PCMX), dnCSL, or dnMaml1 (Lower Right). (B) E-cadherin protein expression in MCF7 cells in normoxia (N) or hypoxia (Upper). E-cadherin expression in MCF10 cells grown on recombinant FC domain or recombinant Jagged, to activate Notch signaling, in normoxia and hypoxia in the presence of absence of GSI as indicated (Lower).