Fig. 3.

GC loss up-regulates dilp message but activates expression of dFOXO target genes. (A–D) GC-less flies (UASp-bam⁺/+; nos-GAL4::VP16/+) exhibit increased production of dilp 2, dilp 3, and dilp 5, both in the y w (A–C) and w¹¹¹⁸ backgrounds (D–F), relative to controls. (G–H) GC loss causes up-regulation of dFOXO targets 4E-BP (G) and l (2)efl (H) in both backgrounds. For details of genotypes, see Fig. 1.