. 2006 Nov 14;4(13):183–191. doi: 10.1098/rsif.2006.0174

Table 3.

Fluorescence anisotropy decay of pyruvate kinase from Bacillus acidocaldarius in the absence and presence of Na⁺ ions. Emission and excitation were set at 340 and 290 nm, respectively.

Na⁺	0 mM		80 mM
		0.47 (0.02)^a
τ₁ (ns)^b		2.6 (0.1)^a
τ₂ (ns)^b		6.7(0.2)^a
τ₃ (ns)^b
α₁	0.314 (0.009)^a		0.567 (0.008, 0.007)^a
α₂	0.47 (0.01)^a		0.347 (0.007)^a
α₃	0.21 (0.02)^a		0.086 (0.008, 0.009)^a
ϕ₁ (ns)	0.038 (0.003)^a		0.056 (0.003)^a
ϕ₂ (ns)	26.5 (1.1)^a		26.2 (1.5)^a
r₀^c	0.28		0.28
g₂	0.428 (0.002)^a		0.399 (0.003)^a
Χ²^d_R	1.05		1.57

Standard deviations were estimated according to D'Auria et al. (2000b).

Global parameters from fitting of 15 frequency-domain decay curves.

A fixed parameter for λ_exc=297 nm.

For δθ=0.2° and δm=0.005.