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. 2008 May 14;3(5):e2152. doi: 10.1371/journal.pone.0002152

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Suh et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Cartoon of the two-component fat body enhancer trap screen. (B) Schema of the longevity screen. (C) C32 (minimal promoter Gal4); UAS-GFP larvae were examined and photographed under GFP fluorescent microscopy, which showed strong expression in the fat body. (D) Adult control or C32; UAS-GFP flies were photographed under bright field (BF) or GFP fluorescent microscopy. Arrow indicates the abdomen, which contains much of the adult fat body. Slight background fluorescence in control fly is due to the presence of the yeast, which is used as a food source, in the gut. (E) The fat body of C32; UAS-GFP larvae or adults was removed, incubated with the fat specific fluorescent dye Nile Red and photographed under bright field (BF) as well as GFP and Nile Red fluorescent microscopy. (F) The intestinal tract of C32; UAS-GFP larvae were removed and then examined and photographed with GFP fluorescent microscopy, which showed that portions of the anterior midgut (segment between arrows) express GFP.