Figure 4. Molecular mapping of the cleavage site of E-cadherin by meprinβ.
(A) Schematic representation of E-cadherin and antibody epitopes. a: DECMA, b: SC7870, c: BDclone 36. S: Signal peptide, Pro: Propeptide, EC: Cadherin-domain, TM: transmembrane domain. (B) Protein extracts from MDCKwt and MDCKαβ cell lines were resolved by SDS-PAGE and analyzed by immunoblot using antibodies a–c. The same membrane was successively stripped and reprobed.