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. Author manuscript; available in PMC: 2009 Feb 15.
Published in final edited form as: Anal Biochem. 2007 Sep 19;373(2):330–336. doi: 10.1016/j.ab.2007.09.015

Fig. 3. Comparison of anti-tau monoclonal antibodies for detection of tau aggregates.

Fig. 3

The dilution series described in Fig. 1 was vacuum-filtered through a 0.2 μm nitrocellulose membrane. Net chemiluminescence was quantified using Tau1 (A), Tau5 (B), or Alz50 (C) primary antibodies and appropriate HRP-conjugated secondary antibodies (goat anti-mouse IgG for Tau1 and Tau5, goat anti-mouse IgM for Alz50) and plotted versus relative tau filament concentration (undiluted analyte = 1). Each data point represents the mean ± SD of triplicate measurements, whereas the solid curves represent best fits of the data points to a power function (eq. 1). All primary antibodies yielded non-linear concentration curves.