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SDS-PAGE analysis of VH and VL proteins folding into dsFv. M: protein molecular weight markers; 1: dsFv boiled for 5 min; 2: dsFv boiled for 10 min; 3: dsFv treated at 37°C for 15 min; 4: VH and VL proteins folding into dsFv; 5: VH and VL proteins diluted into refolding buffer; 6: VL protein; 7: dsFv incubated with β-mercaptoethanol; 8: VH protein.
