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. 2007 May 8;96(10):1595–1604. doi: 10.1038/sj.bjc.6603755

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Copyright © 2007 Cancer Research UK

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RES does not significantly affect p85/PI3K but decreases AR and ERα levels in LNCaP and PC-3 cells. LNCaP (A, C) and PC-3 (B, D) were left untreated (0, DMSO) or treated with 1, 10, 50, 100 or 150 μM RES for 36 h. Total protein extracts were obtained and analysed for p85/PI3K (A, B), AR (C) or ERα (D) protein expression by Western immunobloting using specific antibodies. The level of β-actin was also determined to account for protein quantitation and equal loading. p85/PI3K, AR and ERα expression were quantitated and normalised by β-actin in at least three different cultures. Data shown are mean±s.e. The difference with respect to untreated cultures (DMSO) is significant at P<0.05 (^*).