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. 2007 May 8;96(10):1595–1604. doi: 10.1038/sj.bjc.6603755

Figure 3.

Figure 3

The AR-associated PI3K activity is modulated by RES in androgen-sensitive LNCaP cells. (A) LNCaP cells were cultured for 5 days in steroid-depleted medium and then treated with DMSO (control), 10−9 M of the AR agonist DHT (30 min), 10−9 M DHT (30 min) plus 10−4 M of the AR antagonist Bic (30 min) or 150 μM RES (30 min). Aliquots of 1 mg total cell extract were immunoprecipitated with an AR-specific antibody and PI3K activity determined in the immunoprecipitates using PI as substrate. (B) PI3K activity was determined in AR immunoprecipitates obtained from cultures grown in steroid-depleted medium and left untreated (DMSO) or treated with 1, 10, 50, 100 or 150 μM RES for 36 h. Some cultures were treated with 20 μM of the PI3K inhibitor LY294002 (LY). (C) PI3K activity was also determined in AR immunoprecipitates from cultures grown in complete medium and treated with 0 (DMSO), 1, 10, 50, 100 or 150 μM RES. Androgen-insensitive PC-3 cells (PC3) were used as negative control. A representative experiment out of two is shown.