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. 2007 May 8;96(10):1595–1604. doi: 10.1038/sj.bjc.6603755

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Copyright © 2007 Cancer Research UK

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The effects of RES on p85/PI3K and PKB/AKT phosphorylation in cultured human primary prostate tumour cells closely resemble those observed in LNCaP and PC-3 cells. A total of seven biopsies from patients scheduled for radical prostatectomy were used to obtain primary cultures of prostate epithelial tumour cells. Cells were characterised as having a transformed phenotype as previously were indicated (Sanchez et al, 2005; Castellon et al, 2006, 2005). Cultures treated with DMSO, 10, 50 or 100 μM RES for 36 h in complete medium. (A) Total protein extracts were analysed for p85/PI3K expression by Western immunobloting and the results normalised by β-actin levels. (B) Phosphorylated (pAKT) and total PKB/AKT (tAKT) were also determined by Western immunobloting and the amount of active protein (pAKT) normalised by the total PKB/AKT (tAKT). Data are shown as mean±s.e. The differences with respect to untreated control cultures are significant at P<0.01 (^**).