Figure 2.

(A) Western blot analysis of protein extracts from ovarian tumours. Sample numbers are the same as those shown in Figure 1A. The same blot is shown having been exposed sequentially to eEF1A2 and GAPDH antibodies with a stripping step in between each antibody. The controls are HeLa, which expresses both eEF1A1 and eEF1A2, lymphoblastoid cells (LB) which express only eEF1A1, and adult mouse muscle, which expresses only eEF1A2. Clear-cell tumours are indicated by bold underlined type. (B) Western blot analysis of protein extracts from ovarian cancer cell lines. The negative control is a lymphoblastoid cell line and the positive controls are HeLa, which we have previously found to express eEF1A2 at high levels, and mouse muscle.