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. 2007 Jun 12;96(12):1849–1854. doi: 10.1038/sj.bjc.6603824

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Copyright © 2007 Cancer Research UK

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Methylcholanthrene-induced tumours exhibit a significant enrichment of CD4⁺FOXP3⁺ Tregs. Sections of MCA-induced tumours were stained with anti-FOXP3-specific Ab and either anti-CD4 (A) or –CD8 (B) specific mAb. Single-cell suspensions of TILs, NTDLN, TDLN, spleen and blood were stained with anti-CD4-, anti-CD25- and anti-FOXP3-specific mAbs and analysed by flow cytometry. A representative FACS plot of TIL staining is shown and CD4⁺FOXP3⁺ cells were gated (R2) (C). This gate was used to examine CD25 expression as shown in the right panel (D). The percentage of CD4⁺ cells expressing FOXP3 in each compartment is presented (E). Data were analysed using a paired student's t-test.