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. 2007 May 22;96(12):1862–1870. doi: 10.1038/sj.bjc.6603806

Figure 1.

Figure 1

Immunohistochemical and immunofluorescence staining with L19-SIP antibody in SW1222 and LS174T xenograft-bearing mice. (A) Immunohistochemical staining of SW1222 xenograft lines with L19-SIP antibody. (B–D) Multiple digital fluorescence images of an LS174T tumour injected with Cy3-labelled L19-SIP antibody for 6 h demonstrating (B) perfusion, (C) blood vessel staining and (D) L19-SIP in the same tumour section. (E) Triple fluorescence staining of CD31 (green), L19-SIP (red) and DAPI (blue) in LS174T tumours. (F) High-power image of CD31 (green), L19-SIP (red) and Hoechst (blue) staining in LS174T (arrow indicating abluminal localisation of the L19-SIP antibody). All images are at × 20 magnification except (F) which is at × 40 magnification.