Figure 3.

Time courses of event latency and fluorescence. (A) Time course of (a) mean total ΔF/F₀ averaged over each scan line of the four images used for Fig. 2. (b) Component of total ΔF/F₀ enclosed within the boxes of all identified events. (c) “Nonevent + residual” fluorescence, calculated as a minus b. (d) Estimation of the residual fluorescence remaining after terminating the test pulse (▪ and thin line, records not shown; c repeated as broken line). (e) Nonevent fluorescence related to release, calculated as c minus thin line in d. (B) (a) Histogram of latency from the start of the depolarization of all identified events. (b) Rate of SR Ca²⁺ release calculated as the convolution of the latency histogram in a with either the corresponding rise time of each individual event (solid line) or with the average rise time of all events superimposed (broken line). (c) Histogram of the first latencies of events in each triad along the scan line. All records were normalized to the same peak value, with the normalization factor the same for a and c. (C) Records from Ba, Bb, and Aa (peaking in that order) displayed superimposed on an expanded time scale. Note the different time scales in A–C.