Table 3. Relative hENT1, dCK, RRM1, and RRM2 mRNA expression to GAPDH in human pancreatic cancer cell lines.
|
Relative mRNA expression to GAPDH
|
|||||
|---|---|---|---|---|---|
| Cell lines | IC50 (nM) | hENT1 | dCK | RRM1 | RRM2 |
| PCI43 | 350 | 1.00±0.12 | 0.36±0.0.3 | 0.43±0.02 | 0.50±0.04 |
| PK1 | 160 | 1.00±0.13 | 1.00±0.16 | 1.00±0.13 | 1.00±0.21 |
| KLM1 | 80 | 3.88±0.22 | 0.81±0.02 | 1.08±0.04 | 1.46±0.13 |
| PK9 | 70 | 1.12±0.17 | 0.99±0.05 | 0.31±0.02 | 0.63±0.06 |
| PK8 | 40 | 3.45±0.28 | 2.51±0.07 | 0.71±0.04 | 1.82±0.19 |
| MIAPaCa2 | 40 | 2.64±0.42 | 0.42±0.08 | 0.42±0.07 | 0.47±0.04 |
| KPIN | 40 | 23.26±1.33 | 2.59±0.04 | 1.58±0.02 | 4.31±0.41 |
| BxPC3 | 30 | 3.52±0.51 | 1.20±0.21 | 0.70±0.08 | 0.84±0.16 |
| P-value to IC50 | NS | NS | NS | NS | |
NS: not significant.
Abbreviations: dCK=deoxycytidine kinase; GAPDH=glyceraldehyde-3-phosphate dehydrogenase; hENT1=human equilibrative nucleoside transporter-1.