Figure 2.

Activation of caspase 8, caspase 3 and BID in response to treatment with PL. (A) After treating with different concentrations of PL, cell lysates from PrEC, LNCaP and PC3 cells were prepared. Expression of the activated form of caspase 8 was determined by Western blot. Equal loading of total proteins was verified by β-actin expression. (B) After PL treatment, cell lysates were prepared to analyse caspase 8 activity (B–D). Following the treatments, lysates were prepared to analyse the presence of the cleaved, active forms of caspase 3 or BID. Equal loading of total proteins was verified by β-actin expression.