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. 2007 Mar 6;96(5):769–775. doi: 10.1038/sj.bjc.6603639

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Copyright © 2007 Cancer Research UK

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Changes in TS mRNA and protein expression after treatment with actinomycin in parental wt p53 (Lovo 92) and transfected mt p53 (Lovo 175X2) colon cancer cells. Analysis of (A) mRNA and (B) protein expression were performed with real time with quantitative PCR and Western blot, respectively. (C) Representative plot of Western blot analyses performed as described in the Material and Methods. Significant differences (P<0.05) between control and treated cells are indicated with ^*.