Table 2. Relationship between MMPs and TIMPs immunostaining score values and clinico-pathological characteristics in 131 patients with invasive ductal carcinoma of the breast.
| Characteristics | No. | MMP-1 | MMP-2 | MMP-7 | MMP-9 | MMP-11 | MMP-13 | MMP-14 | TIMP-1 | TIMP-2 | TIMP-3 |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Total cases | 131 | 134 (0–285) | 0 (246) | 124 (0–270) | 72 (0–273) | 148 (0–279) | 61 (0–234) | 107 (0–261) | 144 (0–285) | 115 (0–243) | 110 (0–272) |
| Age (years) | |||||||||||
| ⩽58 | 70 | 140 (20–285) | 0 (0–207) | 119 (0–270) | 0 (0–264) | 165 (0–279) | 63 (0–180) | 84 (0–261) | 146 (0–285) | 108 (0–243) | 126 (0–272) |
| >58 | 61 | 140 (35–285) | 0 (0–246) | 145 (0–267) | 72 (0–273) | 147 (0–276.8) | 59 (0–234) | 81 (0–184.9) | 136 (0–273) | 118 (0–243) | 75 (0–264) |
| Menopausal status | |||||||||||
| Premenopausal | 39 | 129 (20–285) | 23 (0–207) | 102 (0–270) | 69 (0–176) | 157 (0–277) | 63 (0–180) | 81 (0–261) | 134 (0–285) | 78 (0–231) | 135 (0–261) |
| Postmenopausal | 92 | 140 (27–285) | 0 (0–246) | 132 (0–267) | 73 (0–273) | 156 (0–279) | 60 (0–234) | 85 (0–258) | 149 (0–282) | 120 (0–243) | 110 (0–272) |
| Tumoral size | P<0.05 | ||||||||||
| T1 | 63 | 131 (20–285) | 0 (0–198) | 119 (0–258) | 65 (0–273) | 144 (0–279) | 66 (0–192) | 81 (0–231) | 134 (0–276) | 78 (0–243) | 116 (0–272) |
| T2 | 68 | 142 (27–285) | 0 (0–246) | 131 (0–270) | 79 (0–264) | 163 (0–277) | 58 (0–234) | 86 (0–261) | 150 (0–285) | 123 (0–243) | 103 (0–271) |
| Nodal status | P<0.001 | P<0.005 | |||||||||
| N (−) | 62 | 145 (20–285) | 0 (0–207) | 62 (0–243) | 75 (0–273) | 166 (0–279) | 67 (0–234) | 82 (0–261) | 140 (0–276) | 140 (0–243) | 117 (0–272) |
| N (+) | 69 | 140 (27–285) | 0 (0–246) | 151 (33–270) | 70(0–237) | 152 (0–276) | 56 (0–192) | 84 (0–258) | 144 (0–285) | 108 (0–243) | 96 (0–265) |
| Stage | P<0.001 | P<0.05 | |||||||||
| I | 39 | 139 (20–285) | 0 (0–136) | 62 (0–160) | 69 (0–273) | 152 (0–279) | 67 (0–147) | 81 (0–231) | 134 (0–276) | 77 (0–243) | 118 (0–272) |
| II | 61 | 140 (35–285) | 0 (0–207) | 132 (0–270) | 76 (0–264) | 161 (0–277) | 62 (0–234) | 85 (0–261) | 148 (0–285) | 118 (0–243) | 117 (0–271) |
| III | 31 | 132 (27–285) | 0 (0–246) | 157 (33–262) | 68 (0–237) | 147 (0–273) | 54 (0–192) | 81 (0–258) | 146 (0–282) | 120 (0–243) | 72 (0–260.4) |
| Histological grade | P<0.05 | ||||||||||
| Well Dif. | 36 | 140 (35–277) | 0 (0–246) | 70 (0–247) | 67 (0–264) | 103 (0–263) | 66 (0–234) | 78 (0–184) | 144 (0–270) | 79 (0–243) | 117 (0–261) |
| Mod. Dif. | 64 | 138 (20–285) | 0 (0–207) | 145 (0–258) | 74 (0–273) | 161 (0–279) | 61 (0–192) | 82 (0–261) | 134 (0–276) | 124 (0–243) | 94 (0–271) |
| Poorly Dif. | 31 | 145 (27–285) | 0 (0–134) | 120 (0–270) | 96 (0–180) | 172 (0–273) | 57 (0–136) | 121 (0–258) | 151 (0–285) | 74 (0–243) | 94 (0–272) |
| Nottingham pronostic index | P<0.005 | ||||||||||
| <3.4 | 45 | 142 (20–285) | 0 (0–136) | 65 (0–239) | 69 (0–273) | 151 (0–279) | 67 (0–234) | 83 (0–261) | 138 (0–276) | 79 (0–243) | 117 (0–261) |
| 3.4–5.4 | 60 | 138 (33–285) | 0 (0–246) | 135 (0–267) | 74 (0–237) | 158 (0–277) | 61 (0–192) | 81 (0–255) | 138 (0–276) | 128 (0–243) | 122 (0–272) |
| >5.4 | 25 | 140 (27–285) | 47 (0–136) | 139 (33–270) | 82 (0–154) | 159 (0–273) | 56 (0–133) | 147 (0–258) | 146 (0–285) | 85 (0–180) | 71 (0–264) |
| Oestrogen receptors | P<0.01 | ||||||||||
| Negative | 62 | 136 (27–285) | 19 (0–246) | 130 (0–267) | 73 (0–273) | 152 (0–279) | 58 (0–147) | 85 (0–258) | 134 (0–282) | 120 (0–243) | 72 (0–263) |
| Positive | 69 | 140 (20–285) | 0 (0–207) | 120 (0–270) | 72 (0–237) | 160 (0–277) | 63 (0–234) | 81 (0–261) | 146 (0–285) | 90 (0–243) | 137 (0–272) |
| Progesterone receptors | |||||||||||
| Negative | 69 | 135 (27–285) | 0 (0–246) | 129 (0–267) | 75 (0–273) | 155.4 (0–277) | 56 (0–147) | 85 (0–258) | 146 (0–282) | 122 (0–243) | 91 (0–271) |
| Positive | 62 | 140 (20–285) | 0 (0–207) | 126 (0–270) | 70 (0–237) | 160 (0–279) | 63 (0–234) | 81 (0–261) | 136 (0–285) | 72 (0–243) | 118 (0–272) |
| Desmoplastic reaction | P<0.05 | P<0.01 | |||||||||
| No | 45 | 130 (20–285) | 0 (0–207) | 129 (0–267) | 66 (0–273) | 112 (0–277) | 52 (0–180) | 81 (0–261) | 119 (0–273) | 78 (0–243) | 95 (0–272) |
| Yes | 86 | 140 (27–285) | 0 (0–246) | 127 (0–270) | 75 (0–264) | 164 (0–279) | 62 (0–234) | 84 (0–258) | 151 (0–285) | 125 (0–243) | 116 (0–262) |
| Peritumoral inflammation | P<0.01 | P<0.001 | |||||||||
| No | 82 | 140 (20–285) | 0 (0–136) | 120 (0–267) | 69 (0–180) | 152 (0–279) | 61 (0–234) | 79 (0–231) | 137 (0–282) | 112 (0–243) | 121 (0–272) |
| Yes | 49 | 134 (35–285) | 47 (0–246) | 130 (0–270) | 75 (0–273) | 161 (0–276.8) | 60 (0–192) | 154 (0–261) | 150 (0–285) | 118 (0–243) | 74 (0–261) |
| Tumoral advancing edge | P<0.05 | ||||||||||
| Expansive | 56 | 143 (20–285) | 0 (0–136) | 129 (0–262) | 71 (0–264) | 144 (0–279) | 57 (0–180) | 84 (0–261) | 135 (0–276) | 80 (0–243) | 138 (0–272) |
| Infiltrating | 75 | 140 (27–285) | 0 (0–246) | 125 (0–270) | 75 (0–273) | 164 (0–277) | 63 (0–234) | 82 (0–184) | 150 (0–285) | 124 (0–243) | 81 (0–257) |
| Vascular invasion | |||||||||||
| No | 85 | 140 (20–285) | 0 (0–207) | 119 (0–267) | 71 (0–273) | 148 (0–279) | 60 (0–192) | 82 (0–255) | 136 (0–282) | 118 (0–243) | 116 (0–272) |
| Yes | 46 | 136 (40–285) | 0 (0–246) | 142 (0–270) | 74 (0–174) | 164 (0–265) | 63 (0–234) | 84 (0–261) | 149 (0–285) | 111 (0–243) | 100 (0–264) |
Data are expressed as median (range).
Statistical results were corrected applying Bonferroni's correction.
Samples on tissue sections were either insufficient or lost for analysis in three cases for MMP1, two cases for MMP-2, four cases for MMP-7, one for MMP-9, two for MMP-11, one for TIMP-1 and one for TIMP-2. The values shown correspond to the total of cases analyzed for each protein.