Table 1. Analysis of siRNA knockdown with respect to apoptosis and levels of ERdj5 and ERp57 mRNA levels in three cell lines SH-SY5Y, SK-Mel-110 and A375.

Apoptosis:	ERdj5		ERp57
Effect	F	P	F	P
Fenretinide (F1,101)	320	<0.0001	647	<0.0001
SiRNA (F2,101)	23.8	<0.0001	40.2	<0.0001
siRNA dose (F1,101)	4.29	0.041	5.5	0.02
Hypothesis tests
Difference between target siRNAs	0.07	0.79	0.107	0.74
Difference between target siRNA and control siRNA	>34	<0.0001	>57	<0.0001
mRNA levels
Effect	F	P	F	P
Fenretinide (F1,101)	15.6	<0.001	15.7	<0.001
SiRNA (F2,101)	113.2	<0.0001	109.8	<0.0001
siRNA dose (F1,101)	0.79	0.38	1.66	0.2
Hypothesis tests:
Difference between target siRNAs	0.03	0.87	1.99	0.16
Difference between target siRNA and control siRNA	>167	<0.0001	>145	<0.0001

Analysis was done using a General Linear Model (GLM) with, as factors, cell line, fenretinide treatment (no fenretinide or single dose as specified in the legends to Figure 5 and 6) and siRNA type (scrambled control and two different siRNA for both ERdj5 and ERp57); siRNA dose (20 or 40 nM) was included as a linear effect. Separate analyses were performed for ERp57 and ERdj5 with respect to apoptosis and mRNA levels. The table gives the value of the F statistic (degrees of freedom are given for each main effect) and the associated probability (P) for the ERp57 and Erdj5 GLMs. Differences between cell lines in the context of the analysis are trivial and are not reported in the table below.