Table 2. Oligonucleotide primer pairs used during RT–PCR.
| Target gene | Primer sequence 5′ → 3′ |
|---|---|
| HPRT | F GACCAGTCAACAGGGGACAT |
| R CGACCTTGACCATCTTTGGA | |
| PTHrP | F GCTCGGTGGAGGGTCTCA |
| R TTGTCATGGAGGAGCTGATGTT | |
| α 2 | F AACATCCCAGACATCCCAAT |
| R ATCATGTGATTCACCGTCAG | |
| α5 (Oki et al, 2004) | F GGCAGCTATGGCGTCCCACTGTGG |
| R CATCAGAGGTGGCTGGAGGCTT | |
| α 6 | F TCAATTGCTGGAAACATGGA |
| R GGCGGAGGTCAATTCTGTTA | |
| β1 (Oki et al, 2004) | F GTGGTTGCTGGAATTGTTCTTATT |
| R TTTTCCCTCATACTTCGGATTGAC | |
| β4 (Utermark et al, 2003) | F ATAGAGTCCCAGGATGGAGGA |
| R GTGGTGGAGATGCTGCTGTA |
HPRT=hypoxanthine guanine phosphoribosyl transferase; PTHrP=parathyroid hormone-related protein; RT–PCR=reverse transcriptase–polymerase chain reaction.