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. 2006 Dec 19;96(1):126–133. doi: 10.1038/sj.bjc.6603529

Figure 2.

Figure 2

Bmi-1 gene is not amplified in OSCC. Genomic DNAs from five different NHOK strains, two NHOF strains, and seven cancer cell lines were digested with EcoR I and Hind III and transferred for probing. Radiolabelled probes synthesised from Bmi-1 or GAPDH cDNA were hybridised sequentially onto the membrane. The phosphometric intensities were plotted as the ratio of Bmi-1 to GAPDH. The lack of statistical difference (P>0.05) in the levels of Bmi-1 radioactive signals was determined by unpaired T-test (one-ways ANOVA) between the NHOK and the cancer groups.